J.-M. Peters

Cohesin supercoils DNA during loop extrusion

This study shows cohesin supercoils DNA during loop extrusion, influencing genome architecture through both looping and supercoiling.

All eukaryotic SMC proteins induce a twist of -0.6 at each DNA-loop-extrusion step

The paper measures the twist induced by eukaryotic SMC proteins during DNA loop extrusion and finds all three SMC complexes induce a consistent -0.6 twist per step.

Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

This study shows cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase, independent of condensins, suggesting loop extrusion is a general mechanism for genome segregation.

SORORIN is an evolutionary conserved antagonist of WAPL

This study identifies SORORIN orthologs in yeast and plants, showing they antagonize WAPL to maintain cohesion, with tissue-specific functions in plants.

CTCF is a DNA-tension-dependent barrier to cohesin-mediated DNA loop extrusion

The paper shows CTCF blocks cohesin-mediated DNA loop extrusion and regulates its direction and loop size, revealing it as an active regulator rather than a passive barrier.

Can pseudotopological models for SMC-driven DNA loop extrusion explain the traversal of physical roadblocks bigger than the SMC ring size?

This study evaluates pseudotopological models of SMC-driven DNA loop extrusion and finds they conflict with experimental data on roadblock traversal.

SMC complexes can traverse physical roadblocks bigger than their ring size

The paper shows that SMC complexes can extrude DNA loops around large DNA-bound obstacles, suggesting a nontopological mechanism for loop extrusion.

Nanoscale 3D DNA tracing in single human cells visualizes loop extrusion directly in situ

This study uses nanoscale 3D imaging to show that DNA folds as flexible coils without loop extruding enzymes, but forms reproducible loops with Cohesin and CTCF.

MCM complexes are barriers that restrict cohesin-mediated loop extrusion

The paper shows MCM complexes block cohesin-mediated loop extrusion, reducing CTCF-anchored loops and TAD boundary insulation in G1 phase.

Sister-chromatid-sensitive Hi-C reveals the conformation of replicated human chromosomes

This study introduces scsHi-C to analyze sister-chromatid interactions in human chromosomes, revealing frequent contacts at TAD boundaries and dynamic cohesin roles.

Quantifying the heterogeneity of macromolecular machines by mass photometry

This paper demonstrates that mass photometry accurately quantifies sample heterogeneity with high resolution and minimal volume, outperforming negative stain electron microscopy in detecting proteasome assemblies.

A Protein Engineering Approach for Uncovering Cryptic Ubiquitin-binding Sites: from a Ubiquitin-Variant Inhibitor of APC/C to K48 Chain Binding

The paper identifies a cryptic ubiquitin-binding site on APC/C using ubiquitin variants, revealing its preference for K48-linked chains and demonstrating a method for uncovering such sites in large complexes.

Absolute quantification of cohesin, CTCF and their regulators in human cells

The paper measures absolute copy numbers and dynamics of cohesin, CTCF, and related proteins in human cells, revealing that some genomic enrichment sites are unoccupied in single cells.

Dynamics of sister chromatid resolution during cell cycle progression

This study investigates sister chromatid organization in live human cells, finding that sister loci separate during G2 phase and equilibrate within dynamic chromatin polymers, challenging the notion of persistent cohesin sites.

Synthetic lethality between the cohesin subunits STAG1 and STAG2 in diverse cancer contexts

The paper identifies a synthetic lethal relationship between STAG1 and STAG2 in cancers, showing STAG1 inactivation selectively targets STAG2-mutated cancer cells.