Author

Katherine M. Piscopo

Howard Hughes Medical Institute

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Genetics· 1

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Organism:C. elegans· 1 Approach:experimental assay· 1

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Craig C. MelloiD· 1 shared Gregoriy A. DokshiniD· 1 shared Krishna Sumanth GhantaiD· 1 shared

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2018-2018 · peak 1/yr

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Craig C. MelloORCID

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GeneticsGeneticsPeer-reviewedSep 13, 2018

Robust genome editing with short single-stranded and long, partially single-stranded DNA donors in C. elegans

Gregoriy A. Dokshin, Krishna Sumanth Ghanta, Katherine M. Piscopo, Craig C. Mello

This paper describes an optimized CRISPR-based genome editing protocol in C. elegans using ssODN and hybrid donors, achieving efficient and reproducible edits for both short and long constructs.

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C. elegansexperimental assay

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