Craig C. Mello

The paper identifies new germ granule proteins in C. elegans and shows their roles in RNAi and small RNA inheritance, with PINT-1 being critical for siRNA biogenesis.

The paper uses transcriptomics to study PIE-1's role in maintaining germline identity in C. elegans embryos by comparing somatic and germline blastomeres with and without PIE-1.

This study shows HRDE-1 promotes heterochromatin silencing and small-RNA amplification in C. elegans, establishing a self-enforcing mechanism for transgenerational silencing.

This study identifies GLH proteins' role in controlling Argonaute pathway specificity and promoting transgenerational silencing in C. elegans by binding to target mRNAs and amplifying small RNAs.

This study shows that 5' modifications to DNA repair templates enhance precision genome editing efficiency in multiple animal models and human cells.

This study shows that SUMOylation of HDA-1 in C. elegans is required for Argonaute-directed transcriptional silencing by facilitating interactions with the NuRD complex and nuclear Argonaute HRDE-1/WAGO-9.

The paper reports that RNAi-induced relocalization of target RNA to P granules could not be replicated by independent researchers.

The paper shows that defects in Piwi and germ granules in C. elegans lead to reproductive arrest through altered germ granules, not transposon activation, causing sterility.

This study shows that PIR-1 dephosphorylates ppp-RNAs and is essential for 26G-RNA maturation and regulation of gene expression in C. elegans.

This paper shows that melting dsDNA donor molecules before injection improves precision genome editing in C. elegans, enabling high-efficiency HDR for long edits.

The paper shows PIE-1 promotes SUMOylation and activation of HDAC1 in C. elegans oogenesis, enhancing Argonaute-mediated surveillance.

The paper shows GCNA interacts with Topoisomerase II to resolve DNA-protein crosslinks, maintaining genome stability in germline and early embryos.

This paper describes an optimized CRISPR-based genome editing protocol in C. elegans using ssODN and hybrid donors, achieving efficient and reproducible edits for both short and long constructs.

The paper identifies piRNA binding sites in C. elegans germline mRNAs and shows piRNA targeting follows microRNA-like rules, with binding energy more important than piRNA abundance.