Author

L. F. Zacchi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5

Signature Assays, Organisms & Methods

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Assay:proteomics· 5 Approach:experimental assay· 3 Research Focus:cell type annotation· 2 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Approach:statistical model· 2 Research Focus:benchmarking· 1 Approach:deep learning· 1

Frequent Co-Authors

B. L. Schulz· 5 shared C. B. Howard· 4 shared C. L. Pegg· 4 shared D. Roche Recinos· 3 shared T. K. Phung· 3 shared C. Aitken· 2 shared V. Sandford· 2 shared Y. Y. Lee· 2 shared

Activity by Year

2020-2021 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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5 shared papersBiochemistry

B. L. Schulz

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Author profile →

5 papers

Recent·Most cited

N-glycosylation on Oryza Sativa Root Germin-like Protein 1 is conserved but not required for stability or activity

T. Rubbab, C. L. Pegg, T. K. Phung, A. S. Nouwens, B. K. Y. Yeo, L. F. Zacchi, A. Muhammad, S. M. S. S. Naqvi, B. L. Schulz

This study shows that N-glycosylation on OsRGLP1 is conserved but not required for stability or activity, and describes a method for its efficient production in E. coli.

bioRxiv · Feb 9, 2021Biochemistry

Analysis of coagulation factor IX in bioreactor cell culture medium predicts yield and quality of the purified product

L. F. Zacchi, D. Roche Recinos, C. L. Pegg, T. K. Phung, M. Napoli, C. Aitken, V. Sandford, S. M. Mahler, Y. Y. Lee, B. L. Schulz, C. B. Howard

The paper uses DIA-MS proteomics to predict rFIX yield and quality by analyzing bioreactor supernatant, identifying PTMs and their impact on purification and function.

bioRxiv · Jun 3, 2020Biochemistry

Identification of novel glycosylation events on human serum-derived Factor IX

C. L. Pegg, L. F. Zacchi, D. Roche Recinos, C. B. Howard, B. L. Schulz

The paper identifies two novel glycosylation sites on human Factor IX, including an O-linked site at serine 141 and an N-linked site at asparagine 258, contributing to structural characterization for therapeutic development.

bioRxiv · Mar 6, 2020Biochemistry

S-Trap eliminates cell culture media polymeric surfactants for effective proteomic analysis of mammalian cell bioreactor supernatants

L. F. Zacchi, D. Roche Recinos, E. Otte, C. Aitken, T. Hunt, V. Sandford, Y. Y. Lee, B. L. Schulz, C. B. Howard

This study compares sample preparation methods to remove polymeric surfactants from bioreactor supernatants, finding S-Trap provides the best clean-up and data quality.

bioRxiv · Feb 17, 2020Biochemistry

Glycoproteomic measurement of site-specific polysialylation

R. Pelingon, C. L. Pegg, L. F. Zacchi, T. K. Phung, C. B. Howard, P. Xu, M. P. Hardy, C. M. Owczarek, B. L. Schulz

This paper develops a high-throughput glycoproteomics method to measure site-specific polysialylation using LC-ESI-MS/MS after mild acid hydrolysis and desialylation.

bioRxiv · Jan 21, 2020Biochemistry