Analysis of coagulation factor IX in bioreactor cell culture medium predicts yield and quality of the purified product
L. F. Zacchi, D. Roche Recinos, C. L. Pegg, T. K. Phung, M. Napoli, C. Aitken, V. Sandford, S. M. Mahler, Y. Y. Lee, B. L. Schulz, C. B. Howard
The paper uses DIA-MS proteomics to predict rFIX yield and quality by analyzing bioreactor supernatant, identifying PTMs and their impact on purification and function.
bioRxiv · Jun 3, 2020Biochemistry