T. K. Phung

Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content

This paper introduces Golgi-IP, a method to isolate and analyze the human Golgi's proteome, metabolome, and lipidome, revealing novel components and metabolic features.

PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain

This study identifies that PKC isoforms activate LRRK1 by phosphorylating specific residues in the CORB GTPase domain, enhancing kinase activity.

Fine-tuning the Spike: Role of the nature and topology of the glycan shield in the structure and dynamics of SARS-CoV-2 S

This study uses molecular dynamics simulations to show how specific glycosylation sites on SARS-CoV-2 spike protein affect RBD stability and infectivity.

Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Serum N- and O-Glycopeptide Data

This study evaluates glycoproteomics software performance using human serum datasets, identifies high-performance strategies, and provides recommendations for improved analysis.

N-glycosylation on Oryza Sativa Root Germin-like Protein 1 is conserved but not required for stability or activity

This study shows that N-glycosylation on OsRGLP1 is conserved but not required for stability or activity, and describes a method for its efficient production in E. coli.

The post-translational modification landscape of commercial beers

This study analyzes the post-translational modification landscape of 23 commercial Australian beers using DIA/SWATH-MS, revealing high diversity in PTMs and brewery-specific glyco/proteome profiles.

Quantitative data independent acquisition glycoproteomics of sparkling wine

This study quantitatively analyzes glycoproteomics in sparkling wines to assess aging and yeast strain effects on glycosylation profiles.

Analysis of coagulation factor IX in bioreactor cell culture medium predicts yield and quality of the purified product

The paper uses DIA-MS proteomics to predict rFIX yield and quality by analyzing bioreactor supernatant, identifying PTMs and their impact on purification and function.

Proteomics reveals commitment to germination in barley seeds is marked by loss of stress response proteins and mobilisation of nutrient reservoirs

This study uses proteomics to show that barley seed germination involves loss of stress proteins and mobilization of nutrient stores, with differences in dormant seeds.

Glycoproteomic measurement of site-specific polysialylation

This paper develops a high-throughput glycoproteomics method to measure site-specific polysialylation using LC-ESI-MS/MS after mild acid hydrolysis and desialylation.

The intrinsic and regulated proteomes of barley seeds in response to fungal infection

This study quantifies changes in barley seed proteomes under fungal infection using SWATH-MS, finding minimal and variable effects on seed proteins.

Process proteomics of beer reveals a dynamic proteome with extensive modifications

This study uses mass spectrometry proteomics to analyze the dynamic proteome of beer production, identifying significant changes in protein abundance and modifications across process steps.