BiophysicsbioRxiv
Molecular underpinnings of ssDNA specificity by Rep HUH endonucleases and implications for HUH-tag multiplexing and engineering
K. J. Tompkins, M. Houtti, L. A. Litzau…·W. R. Gordon
The paper investigates the molecular mechanism of ssDNA specificity by HUH-endonucleases and demonstrates how this can be engineered for multiplexed HUH-tagging and bioconjugation.
DOI 10.1093/nar/gkaa1248