Molecular underpinnings of ssDNA specificity by Rep HUH endonucleases and implications for HUH-tag multiplexing and engineering
K. J. Tompkins, M. Houtti, L. A. Litzau, E. J. Aird, B. A. Everett, A. T. Nelson, L. Pornschloegl, L. K. Limon-Swanson, R. L. Evans, K. Evans, K. Shi, H. Aihara, W. R. Gordon
The paper investigates the molecular mechanism of ssDNA specificity by HUH-endonucleases and demonstrates how this can be engineered for multiplexed HUH-tagging and bioconjugation.
bioRxiv · Sep 1, 2020Biophysics