Two-target quantitative PCR to predict library composition for shallow shotgun sequencing
M. Y. Cho, M. Oliva, A. Spreafico, B. Chen, X. Wei, Y. Choi, R. Kaul, L. L. Siu, B. Coburn, P. H. H. Schneeberger
The paper develops a two-target qPCR assay to predict human-to-microbe ratios in samples, enabling better planning of shotgun sequencing experiments.
bioRxiv · Sep 21, 2020Microbiology