MicrobiologybioRxiv
Two-target quantitative PCR to predict library composition for shallow shotgun sequencing
M. Y. Cho, M. Oliva, A. Spreafico…·P. H. H. Schneeberger
The paper develops a two-target qPCR assay to predict human-to-microbe ratios in samples, enabling better planning of shotgun sequencing experiments.
DOI 10.1128/msystems.00552-21