L. T. Angenent

The gases H2 and O2 in open-culture reactors influence the performance and microbiota of chain elongation into n-caproate and n-caprylate.

This study investigates how H2 and O2 levels in open-culture reactors affect n-caproate and n-caprylate production and microbiota composition.

Power-to-Vitamins: Producing Folate (Vitamin B9) from Renewable Electric Power and CO2 with a Microbial Protein System

This paper describes a microbial system to produce folate from renewable energy and CO2, achieving 6.7 mg of folate per 100 g biomass with zero or negative net-carbon emissions.

Exclusive D-lactate-isomer production during a reactor-microbiome conversion of lactose-rich waste by controlling pH and temperature

This study shows that controlling pH and temperature in a continuous reactor system can selectively produce D-lactate from lactose-rich waste by manipulating microbial community composition.

An integrated systems-biology platform for power-to-gas technology

This paper develops a systems-biology platform for Methanothermobacter spp. to optimize power-to-gas technology by analyzing genome-scale metabolic models and growth performance in bioreactors.

Carbon oxidation with sacrificial anodes to inhibit O2 evolution in membrane-less bioelectrochemical systems for microbial electrosynthesis

This paper demonstrates a membrane-less bioelectrochemical system using sacrificial carbon anodes to inhibit O2 evolution, enabling efficient microbial electrosynthesis with high methane production rates.

The targeted deletion of genes responsible for expression of the Mth60 fimbriae leads to loss of cell-cell connections in M. thermautotrophicus ΔH

This study deletes genes encoding Mth60 fimbriae in M. thermautotrophicus ΔH, showing a loss of cell-cell connections and altered biofilm formation.

Near-neutral pH increased n-caprylate production in a microbiome with product inhibition of methanogenesis

This study shows that near-neutral pH enhances n-caprylate production in a microbiome by inhibiting methanogenesis through undissociated n-caprylic acid.

Genetic evidence reveals the indispensable role of the rseC gene for autotrophy and the importance of a functional electron balance for nitrate reduction in Clostridium ljungdahlii

This paper uses CRISPR-Cas12a to delete genes in Clostridium ljungdahlii, showing that rseC is essential for autotrophy and that nitrate reduction is linked to energy metabolism.

A shuttle-vector system allows heterologous gene expression in the thermophilic methanogen Methanothermobacter thermautotrophicus ΔH

This paper develops a shuttle-vector system for genetic modification of Methanothermobacter thermautotrophicus, enabling heterologous gene expression and growth on alternative substrates.

Direct medium-chain carboxylic-acid oil separation from a bioreactor by an electrodialysis/phase separation cell

The paper develops an electrodialysis/phase separation cell to directly extract medium-chain carboxylic acid oil from bioreactor broth, achieving higher efficiency than membrane electrolysis with pertraction.

The isolate Caproiciproducens sp. 7D4C2 produces n-caproate at mildly acidic conditions from hexoses: genome and rBOX comparison with related strains and chain-elongating bacteria

This study isolates and characterizes Caproiciproducens sp. 7D4C2, showing it produces n-caproate at mildly acidic pH from hexoses and compares its genome and rBOX genes to related strains.

An open-source multiple-bioreactor system for replicable gas-fermentation experiments: Nitrate feed results in stochastic inhibition events, but improves ethanol production of Clostridium ljungdahlii with CO2 and H2

Food waste as a resource: grinding, dilution, and storage as a pretreatment strategy to produce fermentation intermediates

The paper investigates storage conditions for food waste to optimize fermentation intermediates, finding that temperature, composition, and time significantly affect lactate production.

The short-term effect of residential home energy retrofits on indoor air quality and microbial exposure: a case-control study

This study examines how residential energy retrofits affect indoor air quality and microbial exposure by comparing weatherized and non-weatherized homes.

Suppressing peatland methane production by electron snorkeling through pyrogenic carbon

The paper shows that pyrogenic carbon from fires reduces methane production in peatlands, leading to a net climate benefit by decreasing greenhouse gas emissions.

Syntrophy via interspecies H2 transfer between Christensenella and Methanobrevibacter underlies their global co-occurrence in the human gut

The paper shows that Christensenella and Methanobrevibacter species co-occur in the human gut due to interspecies H2 transfer, supporting a lean BMI association through metabolic interactions.