A rapid, sensitive, scalable method for Precision Run-On sequencing (PRO-seq)
Julius Judd, Luke Wojenski, Lauren M. Wainman, Nathaniel D. Tippens, Edward J. Rice, Alexis Dziubek, Geno J. Villafano, Erin M. Wissink, Philip P. Versluis, Lina Bagepalli, Sagar R. Shah, Dig Bijay Mahat, Jacob M. Tome, Charles G. Danko, John T. Lis, Leighton J. Core
A precision run-on sequencing (PRO-seq) variant with one streptavidin bead binding and on-bead enzymatic steps yields adapter-ligated cDNA in about 10 h and libraries from 0.05–0.25 × 10^6 cells.
bioRxiv · May 18, 2020 · PreprintGenomics✦ In-depth digest