Dig Bijay Mahat

This study shows mutant p53R172H in pancreatic cancer enhances immunosuppressive chemokine Cxcl1 expression via enhancer activation, reducing immune checkpoint inhibitor efficacy.

This study introduces scGRO-seq, a single-cell nascent RNA sequencing method using click-chemistry, revealing coordinated transcription and enhancer-gene synchrony.

A precision run-on sequencing (PRO-seq) variant with one streptavidin bead binding and on-bead enzymatic steps yields adapter-ligated cDNA in about 10 h and libraries from 0.05–0.25 × 10^6 cells.

This study shows that heat shock induces transcriptional memory in cells, accelerating promoter-proximal pause-release and slowing termination over mitotic divisions.