Author

Luke Nightingale

The Francis Crick Institute

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioinformatics· 3 Neuroscience· 1 Systems Biology· 1

Signature Assays, Organisms & Methods

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Organism:human· 4 Research Focus:benchmarking· 2 Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Assay:imaging· 2 Approach:machine learning (classical)· 2 Assay:proteomics· 2 Research Focus:clustering· 1

Frequent Co-Authors

Amy StrangeiD· 4 shared Andrea CairoliiD· 2 shared David HouseiD· 2 shared Emma E. CawoodiD· 2 shared George S. BiggsiD· 2 shared Harry WildersiD· 2 shared Ioannis G. RiziotisiD· 2 shared Jacob T. BushiD· 2 shared

Activity by Year

2021-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 37 mutual co-author links

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4 shared papersBioinformatics

Amy StrangeORCID

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Author profile →

5 papers

Recent·Most cited

Determinants of cysteine coverage by chemoproteomics

Ioannis G. Riziotis, Harry Wilders, Andrea Cairoli, Hugues Lambert, Jamie Dark, George S. Biggs, Emma E. Cawood, William J. McCarthy, Luke Nightingale, María Paula Magariños, Mike Gavrielides, Karen Ambrose, Amy Strange, Mark J. Skehel, Simon J. Boulton, , Jacob T. Bush

This study identifies factors governing cysteine detection in chemoproteomics using machine learning and statistical filtering, showing that peptide properties and abundance are key determinants.

bioRxiv · Oct 19, 2026 · PreprintBioinformatics

Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics

George S. Biggs, Emma E. Cawood, Aini Vuorinen, William J. McCarthy, Harry Wilders, Ioannis G. Riziotis, Antonie J. van der Zouwen, Jonathan Pettinger, Luke Nightingale, Peiling Chen, Andrew J. Powell, David House, Simon J. Boulton, Mark J. Skehel, Katrin Rittinger, Jacob T. Bush

This paper develops a label-free chemoproteomics platform to profile cysteine-reactive fragments, identifying over 400 ligand-protein interactions in two cell lines.

Nature Communications · Jan 2, 2025Peer-reviewedSystems Biology

Asymmetric Contrastive Objectives for Efficient Phenotypic Screening

Luke Nightingale, Joseph Tuersley, Scott Warchal, Andrea Cairoli, Jacob Howes, Cameron Shand, Andrew J. Powell, Darren V.S. Green, Amy Strange, Michael T. Howell

This paper introduces asymmetric contrastive objectives for phenotypic screening, improving performance on benchmark datasets and real-world cell screening tasks.

bioRxiv · Apr 19, 2024 · PreprintBioinformatics

OptoPi: An open source flexible platform for the analysis of small animal behaviour

Xavier Cano-Ferrer, Ruairí J.V. Roberts, Alice Sarah French, Joost de Folter, Hui Gong, Luke Nightingale, Amy Strange, Albane Imbert, Lucia L. Prieto-Godino

OptoPi is an open-source platform for small animal behaviour analysis with custom illumination and optogenetic stimulation, demonstrating tracking of zebrafish and Drosophila larvae.

HardwareX · Jun 19, 2023Peer-reviewedNeuroscience

Automatic instance segmentation of mitochondria in electron microscopy data

Luke Nightingale, Joost de Folter, Helen Spiers, Amy Strange, Lucy Collinson, Martin L. Jones

The paper presents a method for automated 3D mitochondria segmentation in electron microscopy data, achieving high performance on rat and human cortex samples.

bioRxiv · May 24, 2021 · PreprintBioinformatics