Kinetics and Evolution-guided Design of High Fidelity CRISPR-Cas Endonucleases
Huihui Liu, Z Hong Zhou, Luowei Yuan, Bin Pang, Kun Xi, X F Li, Wenzhuo Ma, Rujuan Ti, Li Li, Nuo Chen, Yang Xu, Jie Yang, Yiping Yu, Yuchen Yang, Ruobing Ren, , Lizhe Zhu
This study designs high-fidelity CRISPR-Cas variants using a physics-driven approach combining molecular dynamics and protein language models, achieving significant improvements in fidelity with minimal experimental validation.
bioRxiv · Jul 7, 2026 · PreprintBiophysics