Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+
R. S. Zou, Y. Liu, O. E. Reyes Gaido, M. F. Konig, B. J. Mog, L. L. Shen, F. Aviles-Vazquez, A. Marin-Gonzalez, T. Ha
DISCOVER-Seq+ improves CRISPR off-target detection sensitivity by using DNA-PKcs inhibition and ChIP-seq to identify more off-target sites in cells and mice.
bioRxiv · Oct 29, 2022Bioengineering