Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein
S. H. Chan, L. Tirole, D. W. Kneller, T. M. Kelley, N. Dai, G. B. Robb
This paper describes an RNA capping enzyme-T7 RNA polymerase fusion protein that efficiently generates capped synthetic mRNA in a one-step process, achieving up to 95% Cap-1 incorporation.
bioRxiv · Oct 28, 2023 · PreprintBiochemistry