Combined Selective Plane Illumination Microscopy and FRAP maps intranuclear diffusion of NLS-GFP
C. M. Hobson, E. T. O'Brien, M. R. Falvo, R. Superfine
This paper introduces SPIM-FRAP, a technique combining selective plane illumination microscopy and FRAP to map intranuclear diffusion of NLS-GFP, revealing spatial heterogeneity and validating its accuracy against established methods.
Biophysical Journal · Aug 1, 2020Peer-reviewedBiophysics