A one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging
Kilian Roßmann, Ulrich Pabst, Bianca C. Baciu, Siqi Sun, Christiane Huhn, Christina Holmboe Olesen, Maria Kowald, E. Tapp, Marie Bieck, Ramona Birke, Brenda C. Shields, Pyeonghwa Jeong, Jiyong Hong, Michael R. Tadross, Joshua Levitz, , Johannes Broichhagen
The paper introduces a one-step method to create cell-impermeable fluorescent HaloTag substrates for live-cell imaging and super-resolution microscopy.
Nature Communications · Jan 12, 2026Peer-reviewedPharmacology & Toxicology