Christina Holmboe Olesen

HaloTag Ligand and HaloTag Protein engineering for a binary fluorescent turn-on probe

The paper designs a binary fluorescent turn-on probe using a HaloTag mutant and a difluorinated xanthene ligand for covalent labeling and imaging applications.

A bio-orthogonal and covalent 5 kDa small protein tag

The paper introduces a new protein labelling technology called CLUSTER that enables efficient and minimally invasive protein labeling in living cells using a bio-orthogonal approach.

A one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging

The paper introduces a one-step method to create cell-impermeable fluorescent HaloTag substrates for live-cell imaging and super-resolution microscopy.

Structure of the hepatitis C virus E1/E2 envelope proteins in a homodimeric complex

The paper determines the structure of HCV E1/E2 envelope proteins in a homodimeric complex using cryo-electron microscopy, revealing details about the dimer interface and antigenic sites relevant to vaccine design.