Kinetic profiling of metabolic specialists demonstrates stability and consistency of in vivo enzyme turnover numbers
David Heckmann, Anaamika Campeau, Colton J. Lloyd, Patrick V. Phaneuf, Ying Hefner, Marvic Carrillo-Terrazas, Adam M. Feist, David J. Gonzalez, Bernhard Ørn Palsson
The paper estimates in vivo enzyme turnover numbers (kcats) in E. coli using metabolic specialist strains and shows their stability and predictive power over in vitro kcats.
Proceedings of the National Academy of Sciences · Sep 1, 2020Peer-reviewedSystems Biology