Multi-barrier unfolding of the double-knotted protein, TrmD-Tm1570, revealed by single-molecule force spectroscopy and molecular dynamics
Fernando Bruno da Silva, Szymon Niewieczerzał, Iwona Lewandowska, Mateusz Fortunka, Maciej Sikora, Laura‐Marie Silbermann, Katarzyna M. Tych, Joanna Ida Sulkowska
This study uses single-molecule force spectroscopy and molecular dynamics to analyze the unfolding and folding of double-knotted and single-knotted proteins, revealing that double-knotted proteins require chaperone assistance for full knotting.
bioRxiv · Aug 27, 2025 · PreprintBiophysics