Widespread DNA off-targeting confounds studies of RNA chromatin occupancy
Micah Jonathan Goldrich, Louis Delhaye, Sarah-Lee Bekaert, Bieke Decaesteker, Filip Van Nieuwerburgh, Frank Speleman, Sven Eyckerman, Pieter Mestdagh, Igor Ulitsky
The paper shows that many reported RNA-chromatin interactions are technical artifacts caused by DNA off-targeting, not true biological interactions.
Nature Biotechnology · May 15, 2026Peer-reviewedMolecular Biology