CRISPR-Mediated Linearization of IDLV Donor Enables Precise Targeted Integration in Human Hematopoietic Stem Cells
Giulia Scalisi, Aboud Sakkal, Laurie Lacombe, Francesco Sarnari, Marine Rouillon, Michela Rosiello, Alexandra Tachtsidi, Paola Galbiati, Guillaume Corre, Julie Oustelandt, Giulia Pavani, Marine Laurent, Mike A. Firth, Marie As, Marcello Maresca, , Mario Amendola
This paper introduces TILV, a CRISPR-assisted method for precise targeted integration of transgenes in human hematopoietic stem cells using IDLV donors, showing improved efficiency and minimal impact on cell viability.
bioRxiv · Jun 17, 2026 · PreprintMolecular Biology