BiophysicsbioRxivPreprint
Suitable use of FRET-based Biosensors for Quantitative Detection of GPCR Activation
N. K. Brinkenfeldt, A. Dias, J. M. Mathiesen, K. L. Martinez
The paper quantifies cAMP production using a genetically encoded biosensor in living cells and shows that biosensor expression levels affect signal linearity, necessitating stable cell lines for accurate high-throughput screening.
DOI 10.1101/2022.06.01.494354