Modular Engineering of Thermo-Responsive Allosteric Proteins
Kira H Hoffmann, Ann-Sophie Helene Kroell, Nikolas Alexander Motzkus, Nina Lemmen, Nele Happ, Benedict Wolf, Adrian Bachmann, Nicholas T. Southern, Felicitas Vogd, Sabine Aschenbrenner, Dominik Niopek, Jan Mathony
This paper engineers thermo-responsive allosteric proteins using temperature-sensitive domains, enabling precise control of protein activity in both bacterial and mammalian systems.
bioRxiv · May 2, 2025 · PreprintSynthetic Biology