CAGE-TRX expands the scope of time-resolved crystallography through genetically encoded active-site photocaging
Clyde A. Smith, Ailiena O. Maggiolo, Chasity P. Janosko, Maura E. Charette, Nilakshi Paul, Márta Tóth, Guillermo A. Calero, Sierra M Carr, Silvia Russi, Sergei B. Vakulenko, Alexander Deiters, Aina E. Cohen
The paper introduces CAGE-TRX, a method for time-resolved crystallography that uses photocaged amino acids to control enzyme activity and capture reaction intermediates.
bioRxiv · Jun 29, 2026 · PreprintBiochemistry