P. Guptasarma

Differences in the activities of domain-swapped chimeras of two homologous GH57 glucanotransferases suggest that a glucan-binding DUF could influence donor substrate specificity

The paper shows that domain 3 of GH57 glucanotransferases determines donor substrate specificity through a glucan-binding site at its domain interface.

A glucanotransferase that uses two sub-sites and four catalytic aspartates/glutamates to disproportionate oligosaccharides ranging in length from maltotriose to starch

This paper demonstrates that PfuAmyGT is an exo-amylase-cum-glucanotransferase capable of transferring glucose and disproportionating oligosaccharides using separate sub-sites for donor and acceptor binding.

Nucleoid-Associated Proteins Undergo Liquid-Liquid Phase Separation with DNA into Multiphasic Condensates Resembling Bacterial Nucleoids

The paper shows NAPs like HU-A, HU-B, and Dps form multiphasic condensates with DNA, resembling bacterial nucleoids through phase separation.

Enhancing high-temperature degradation of polyethylene terephthalate through a synergistic division of enzyme labour between a solid-degrading thermostable cutinase and a reaction intermediate-degrading thermostable carboxylesterase

This paper demonstrates that combining a solid-degrading cutinase with a solution-degrading carboxylesterase enables complete degradation of PET hydrolysis products into TPA at 60°C, even with residual solid PET.

N-terminal extensions strengthen hydrophobic inter-subunit interactions between HU's C-terminal domains to frustrate heterodimer formation

The paper shows that N-terminal extensions in HU proteins reduce heterodimer formation by stabilizing hydrophobic interactions in the C-terminal domains.

Mechanism of bacterial adhesion and embedment in a DNA biofilm matrix:Evidence that binding of outer membrane lipopolysaccharide (LPS) to HU is key

The paper identifies HU's role in bacterial adhesion to DNA in biofilms by binding LPS to HU's DNA-binding sites, facilitating bacterium-bacterium and bacterium-DNA interactions.

Sustained exposure to trypsin causes cells to transition into a state of reversible stemness that is amenable to transdifferentiation

This paper shows that sustained trypsin exposure induces cells to transition into a reversible stem-like state, enabling transdifferentiation and altering cellular behavior.