Author

P. J. Spreacker

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 2 Biochemistry· 1 Biophysics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:host-pathogen & immune response· 3 Organism:E. coli· 2 Approach:biophysical modeling· 1 Research Focus:drug response / drug discovery· 1 Assay:protein structure· 1 Assay:proteomics· 1

Frequent Co-Authors

K. A. Henzler-Wildman· 4 shared C. J. Porter· 3 shared W. F. Beeninga· 3 shared M. Brousseau· 2 shared A. Wegrzynowicz· 1 shared B. L. Young· 1 shared E. N. Powers· 1 shared G. S. Hisao· 1 shared

Activity by Year

2021-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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4 shared papersMicrobiology

K. A. Henzler-Wildman

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4 papers

Recent·Most cited

Functional promiscuity of small multidrug resistance transporters from Staphylococcus aureus, Pseudomonas aeruginosa, and Francisella tularensis

P. J. Spreacker, C. J. Porter, A. Wegrzynowicz, W. F. Beeninga, S. P. Demas, E. N. Powers, K. A. Henzler-Wildman

The paper tests whether functional promiscuity of SMR transporters extends to homologs from three pathogens, finding they can confer resistance or susceptibility to different substrates in E. coli.

bioRxiv · Nov 22, 2022 · PreprintMicrobiology

Charge neutralization of the active site glutamates does not limit substrate binding and transport by EmrE

P. J. Spreacker, M. Brousseau, G. S. Hisao, M. Soltani, J. Davis, K. A. Henzler-Wildman

The paper tests whether charge neutralization of glutamates limits EmrE's substrate binding and transport, finding it does not.

bioRxiv · Aug 17, 2022 · PreprintBiophysics

Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility

P. J. Spreacker, N. E. Thomas, W. F. Beeninga, M. Brousseau, C. J. Porter, K. M. Hibbs, K. A. Henzler-Wildman

The paper shows that the E. coli SMR transporter EmrE can confer susceptibility to small-molecule substrates like harmane by triggering proton uniport, leading to antibiotic synergy.

bioRxiv · Dec 4, 2021 · PreprintBiochemistry

Functional phenotyping of small multidrug resistance proteins from Staphylococcus aureus and Francisella tularensis reveals functional homology to EmrE

P. J. Spreacker, W. F. Beeninga, B. L. Young, C. J. Porter, K. A. Henzler-Wildman

The paper functionally characterizes EmrE-like SMR transporters from Staphylococcus aureus and Francisella tularensis, showing they are functionally similar to EmrE and distinct from GdX-like SMRs.

bioRxiv · Jan 6, 2021 · PreprintMicrobiology