A Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
P. P. Geurink, R. Kooij, A. Sapmaz, S. Liu, B.-T. Xin, G. M. C. Janssen, P. A. van Veelen, P. ten Dijke, H. Ovaa
This paper develops a fluorescent small-molecule probe to monitor UCHL1 activity in live cells and zebrafish embryos, showing specificity for UCHL1 over other DUBs.
bioRxiv · Nov 1, 2019Biochemistry