S. Benlekbir

Structure and dynamics of a pentameric KCTD5/Cullin3/Gβγ E3 ubiquitin ligase complex

The paper presents the cryo-EM structure of a pentameric KCTD5/CUL3/Gβγ E3 ubiquitin ligase complex, revealing its dynamic architecture and mechanism for ubiquitylating Gβγ.

Structure of a dimeric ABC transporter

This study presents the structure of Ycf1p, a yeast ABC transporter, showing it forms a dimer with distinct phosphorylation and ATPase activity compared to monomers.

Cryo-EM of the yeast VO complex reveals distinct binding sites for macrolide V-ATPase inhibitors

The paper determines the structures of the yeast VO complex bound to two macrolide inhibitors, revealing distinct binding sites on the c ring that block proton translocation.

Structure of Ycf1p reveals the transmembrane domain TMD0 and the regulatory R region of ABCC transporters

This paper presents the cryo-EM structure of Ycf1p, revealing the TMD0 domain and parts of the R region in ABCC transporters, and shows how their orientation and interactions influence protein function and disease.

Electron Event Representation (EER) data enables efficient cryoEM file storage with full preservation of spatial and temporal resolution

The paper introduces EER data format for cryoEM DDD movies, preserving spatial and temporal resolution without increasing file size.

Multiple conformations facilitate PilT function in the type IV pilus

The paper determines four X-ray structures of PilT in different conformations and validates their physiological importance through co-evolution analysis and functional studies.

Shake-it-off: A simple ultrasonic cryo-EM specimen preparation device

The paper introduces a simple ultrasonic cryo-EM specimen preparation device that improves sample transfer efficiency and reduces air-water interface interactions.

Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties

The paper revises the subunit order of mammalian septin complexes, showing they can co-polymerize and revealing an inverted organization compared to previous predictions.