S. Bigot

Real-time visualisation of conjugation reveals the molecular strategy evolved by the conjugative F plasmid to ensure the sequential production of plasmid factors during establishment in the new host cell

The paper uses live-cell microscopy to study conjugation dynamics, revealing that ssDNA plasmid entry and ss-to-dsDNA conversion control sequential gene expression in bacterial plasmids.

Targeted-Antibacterial-Plasmids (TAPs) combining conjugation and CRISPR /Cas systems achieve strain-specific antibacterial activity

This paper develops TAPs, plasmids using conjugation and CRISPR/Cas to kill specific bacterial strains, showing they can resensitize resistant bacteria to antibiotics.