Author

S. Saffarian

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 5 Microbiology· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Research Focus:host-pathogen & immune response· 4 Research Focus:cell signaling & trafficking· 2 Research Focus:cell type annotation· 2 Research Focus:clustering· 2 Organism:human· 2 Research Focus:differential expression· 1 Assay:fluorescence / live-cell microscopy· 1

Frequent Co-Authors

B. Preece· 4 shared I. Saha· 4 shared A. Petersen· 2 shared A. Sharma· 2 shared B. MacArthur· 2 shared H. Durden· 2 shared H. Swann· 2 shared M. Vershinin· 2 shared

Activity by Year

2019-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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4 shared papersBiophysics

B. Preece

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Competitive assembly resolves the stoichiometry of essential proteins in infectious HIV-1 virions.

H. Durden, B. Preece, R. Gallegos, I. Saha, B. MacArthur, A. Petersen, W. Peppel, S. Saffarian

The paper measures the stoichiometry of Gag-Pol and gp120/gp41 proteins in infectious HIV-1 virions using a competitive assembly method, finding it similar to all released virions.

bioRxiv · Mar 10, 2024 · PreprintBiophysics

Defects in the HIV immature lattice support essential lattice remodeling within budded virions

S.-K. Guo, I. Saha, S. Saffarian, M. E. Johnson

This paper uses reaction-diffusion simulations to show that incomplete HIV immature lattice dynamics enable rapid Gag-Pol dimerization, essential for viral maturation.

eLife · Jul 12, 2023Peer-reviewedBiophysics

Competitive HIV budding suggests that a self-packaging gRNA:Gag-Pol complex directs HIV assembly and enforces infectivity.

H. Durden, I. Saha, B. Preece, B. MacArthur, A. Petersen, W. Peppel, R. Gallegos, S. Saffarian

The paper investigates HIV assembly by testing infectious/non-infectious genome mixtures, finding that a gRNA:Gag-Pol complex drives virion formation and infectivity.

bioRxiv · Aug 3, 2022 · PreprintMicrobiology

Optimized production and fluorescent labelling of SARS-CoV-2 Virus-Like-Particles to study virus assembly and entry.

D. Muriaux, M. Gourdelier, J. M. Swain, C. Arone, A. Mouttou, D. Bracquemond, P. Merida, S. Saffarian, S. Lyonnais, C. Favard

This paper optimizes the production of SARS-CoV-2 Virus-Like Particles (VLPs) and demonstrates their fluorescent labeling for studying virus assembly and entry mechanisms.

bioRxiv · Mar 23, 2022 · PreprintMicrobiology

Minimal system for assembly of SARS-CoV-2 virus like particles

H. Swann, A. Sharma, B. Preece, A. Peterson, C. Eldredge, D. Belnap, M. Vershinin, S. Saffarian

The paper demonstrates the assembly of non-infectious SARS-CoV-2 virus-like particles using viral proteins S, M, and E in mammalian cells, showing their structural stability and potential for vaccine development.

Scientific Reports · Dec 14, 2020Peer-reviewedBiophysics

Structural stability of SARS-CoV-2 degrades with temperature

A. Sharma, B. Preece, H. Swann, X. Fan, R. J. McKenney, K. M. Ori-McKenney, S. Saffarian, M. Vershinin

This study shows that SARS-CoV-2 structural stability decreases with temperature, supporting the seasonal pattern of viral infectivity.

bioRxiv · Oct 12, 2020Biophysics

Interferometric Fluorescent Cross Correlation Spectroscopy

I. Saha, S. Saffarian

The paper introduces a method to measure fluorescent molecule transport in aqueous samples using single photon interference and FCS, achieving high-resolution measurements in thousands of voxels.

bioRxiv · Jun 13, 2019Biophysics

Abrogating ALIX interactions results in stuttering of the ESCRT machinery

S. Gupta, M. Bendjennat, S. Saffarian

The paper shows that disrupting ALIX interactions leads to repeated, transient recruitment of ESCRT components during HIV budding, indicating a robust network of interactions that control ESCRT function.

bioRxiv · Mar 14, 2019Cell Biology