Tuning siRNA Specificity through Seed Region Incorporation of Deoxyribonucleotide Stereoisomers
Mehran Nikan, Thazha P. Prakash, Guillermo Vasquez, Graeme C. Freestone, Marie Annoual, Michael Tanowitz, Hongda Li, Sagar S. Damle, Rodrigo Galindo‐Murillo, Stephanie K. Klein, Audrey Low, Clare R. Quirk, Colleen Y. Heller, Dorothy T. Ta, Andrew T. Watt, , Eric E. Swayze
This paper introduces modified nucleosides in siRNA seed regions to enhance specificity and reduce off-target effects while maintaining on-target activity.
bioRxiv · Jan 14, 2026 · PreprintMolecular Biology