DNA targeting by compact Cas9d and its resurrected ancestor
Rodrigo Fregoso Ocampo, Jack P. K. Bravo, Tyler L. Dangerfield, Isabel Nocedal, Samatar Ali Jirde, Lisa M. Alexander, Nicole C. Thomas, Anjali Das, Sarah Nielson, Kenneth Allen Johnson, Christopher T. Brown, Cristina N. Butterfield, Daniela S. Aliaga Goltsman, David Ward Taylor
This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.
Nature Communications · Jan 7, 2025Peer-reviewedBiochemistry