Author

Nicole C. Thomas

ORCID 0000-0002-7200-8015Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Research Focus:protein structure prediction· 2 Research Focus:cancer & tumor microenvironment· 1 Assay:CRISPR screen· 1 Assay:metagenomics· 1 Approach:statistical model· 1

Frequent Co-Authors

Cristina N. ButterfieldiD· 4 shared David Ward TayloriD· 4 shared Christopher T. BrowniD· 3 shared Daniela S. Aliaga GoltsmaniD· 3 shared Lisa M. AlexanderiD· 3 shared Rodrigo Fregoso OcampoiD· 3 shared Tyler L. DangerfieldiD· 3 shared Cindy J. CastelleiD· 2 shared

Activity by Year

2025-2026 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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4 shared papersBiochemistry

Cristina N. ButterfieldORCID

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Structural and kinetic insights into a metagenomics-derived Cas12a with high specificity

Jacquelyn T. Wright, Satya Yendluri, Nicole C. Thomas, Cristina N. Butterfield, Tyler L. Dangerfield, David Ward Taylor

The paper characterizes Cas12a-MG29-1, a metagenomics-derived nuclease, revealing structural and kinetic features that enhance its specificity and activity.

bioRxiv · May 13, 2026 · PreprintBiochemistry

Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophy

Kartik Lakshmi Rallapalli, Joanne M. L. Ho, Isabel Nocedal, Jin Wen Tan, Rodrigo Fregoso Ocampo, Nicole C. Thomas, Brandon Justice, Samatar Jirde, Liliana Gonzalez‐Osorio, Cindy J. Castelle, Jyun‐Liang Lin, Jacquelyn T. Wright, Soungsothira Toch, Krushangi Shah, Benjamin Freeman, , Cristina N. Butterfield

This paper develops compact adenine base editors for in vivo gene editing, demonstrating their application in Duchenne muscular dystrophy by enabling exon skipping through targeted splice-site disruption.

bioRxiv · May 12, 2026 · PreprintBioengineering

Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency

Kaoling Guan, Rodrigo Fregoso Ocampo, Paula B. Matheus Carnevali, Cindy J. Castelle, Liliana Gonzalez‐Osorio, Dominic T. Castanzo, Nicole C. Thomas, Molly Brothers, Tyler L. Dangerfield, Matthew M. Hooper, Madeline S. West, Nathan M. Appleby, Isabella Krudop, Rebecca C. Lamothe, Daniela S. Aliaga Goltsman, , David Ward Taylor

The paper compares Cas12f orthologs to identify mechanistic features that enhance genome editing efficiency in human cells.

Nature Structural & Molecular Biology · Apr 13, 2026Peer-reviewedBiochemistry

DNA targeting by compact Cas9d and its resurrected ancestor

Rodrigo Fregoso Ocampo, Jack P. K. Bravo, Tyler L. Dangerfield, Isabel Nocedal, Samatar Ali Jirde, Lisa M. Alexander, Nicole C. Thomas, Anjali Das, Sarah Nielson, Kenneth Allen Johnson, Christopher T. Brown, Cristina N. Butterfield, Daniela S. Aliaga Goltsman, David Ward Taylor

This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.

Nature Communications · Jan 7, 2025Peer-reviewedBiochemistry