David Ward Taylor

This study identifies a cryptic divalent metal binding pocket that regulates Cas9's metal-dependent activity through conformational activation and catalytic efficiency.

This study investigates how TIGR-Tas systems recognize and cleave DNA targets, revealing asymmetric target engagement and coordinated cleavage across both RNA-DNA heteroduplexes.

The paper presents the cryo-EM structure of AsCas12a bound to a pseudo-DNA guide and RNA target, revealing its mechanism of DNA-guided RNA recognition.

The paper identifies a phage-encoded factor that activates the Hna anti-phage defense system by overcoming its auto-inhibition.

The paper characterizes Cas12a-MG29-1, a metagenomics-derived nuclease, revealing structural and kinetic features that enhance its specificity and activity.

This paper develops compact adenine base editors for in vivo gene editing, demonstrating their application in Duchenne muscular dystrophy by enabling exon skipping through targeted splice-site disruption.

The paper compares Cas12f orthologs to identify mechanistic features that enhance genome editing efficiency in human cells.

The paper identifies a cytoplasmic surveillance pathway that detects and degrades faulty ribosomes during biogenesis but allows defective ribosomes to escape into translation if they bypass the pathway.

The paper identifies and characterizes a new conserved protein complex, CAGE, found in both prokaryotes and eukaryotes, revealing its structural features and evolutionary distribution.

This study uses guide RNA truncation and cryo-EM to visualize multi-turnover Cas9 states, revealing how PAM-proximal product binding inhibits re-binding of new targets.

This paper identifies a structural feature in FnCas9 that enhances DNA targeting fidelity and describes its dual role in gene repression and immune evasion.

This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.

The paper shows Reh1 remains on 60S subunits during early translation elongation and is displaced by the growing peptide chain.

The paper tests if AlphaFold2 accurately models intracellular protein conformations using cross-linking mass spectrometry of endogenous ciliary proteins in Tetrahymena thermophila.

The paper shows that a single 2'-O-methylation of ribosomal RNA is essential for ribosome assembly and nuclear export, with Nog2 interacting specifically with the methylated base.

This study defines the RBC proteome and interactome using mass spectrometry and machine learning, revealing key biological processes and structural models.

The paper shows internal translation of large subunit transcripts generates small subunits in type I CRISPR-Cas complexes, revealing widespread coding potential within cas genes.

The paper introduces a cuboid transformation of protein surfaces to predict interaction interfaces, achieving comparable accuracy to 3D docking methods while being faster and more tolerant to structural changes.

This paper compares genomes of 81 nematode and platyhelminth species to identify gene families related to parasitism and prioritize new drug targets.