Author

Kenneth Allen Johnson

The University of Texas at AustinORCID 0000-0002-6575-2823Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:protein folding & dynamics· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Research Focus:cell-cell communication· 1 Research Focus:differential expression· 1 Organism:human· 1 Approach:mechanistic / dynamical model· 1

Frequent Co-Authors

Tyler L. DangerfieldiD· 3 shared Andrea SchenkmayerováiD· 1 shared Andrew J. deMelloiD· 1 shared Christopher T. BrowniD· 1 shared Ciara Fields· 1 shared Cindy J. CastelleiD· 1 shared Cristina N. ButterfieldiD· 1 shared Daniela S. Aliaga GoltsmaniD· 1 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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3 shared papersBiochemistry

Tyler L. DangerfieldORCID

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Author profile →

4 papers

Recent·Most cited

Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency

Kaoling Guan, Rodrigo Fregoso Ocampo, Paula B. Matheus Carnevali, Cindy J. Castelle, Liliana Gonzalez‐Osorio, Dominic T. Castanzo, Nicole C. Thomas, Molly Brothers, Tyler L. Dangerfield, Matthew M. Hooper, Madeline S. West, Nathan M. Appleby, Isabella Krudop, Rebecca C. Lamothe, Daniela S. Aliaga Goltsman, , David Ward Taylor

The paper compares Cas12f orthologs to identify mechanistic features that enhance genome editing efficiency in human cells.

Nature Structural & Molecular Biology · Apr 13, 2026Peer-reviewedBiochemistry

Transient signaling of free ADP-ribose monitored with an intracellular biosensor

Shivansh Goyal, Vanna Nguyen, Scott N. Lyons, Tyler L. Dangerfield, Wanjie Yang, Ciara Fields, Hsin‐Ru Chan, Yazhini Mahendravarman, Lorren Cantú, Nesha Rubin, Kelton Deloney, Kenneth Allen Johnson, Xiaolu A. Cambronne

The paper develops a biosensor to measure intracellular ADP-ribose dynamics and finds that ADPR levels are transient, tunable, and critical for TRPM2 activation.

bioRxiv · Feb 9, 2026 · PreprintBiochemistry

Conformational gating of product release sets the catalytic ceiling of a bioluminescent reporter

Martin Toul, Jana Horáčková, Andrea Schenkmayerová, Joan Planas-Iglesias, Tomas Landolt, Jeerus Sucharitakul, Yves Louis Janin, Kridsadakorn Prakinee, Pimchai Chaiyen, Stavros Stavrakis, Andrew J. deMello, Kenneth Allen Johnson, Jiřı́ Damborský, Martin Marek, David Bednář, Zbyněk Prokop

This study identifies conformational dynamics as a key determinant of luciferase catalytic efficiency and light-emission stability, revealing a trade-off between oxygen affinity and enzyme longevity.

bioRxiv · Sep 16, 2025 · PreprintBiochemistry

Single-turnover kinetic analysis of non-LTR retrotransposition defines the mechanism and rate constants governing each step

Tyler L. Dangerfield, Jun Zhou, Thomas H. Eickbush, Kenneth Allen Johnson

This study defines the kinetic mechanism and rate constants for non-LTR retrotransposition using single-turnover analysis, revealing the complete reaction sequence and second-strand synthesis.

bioRxiv · Aug 28, 2024 · PreprintBiochemistry