Single molecule co-occupancy of RNA-binding proteins with an evolved RNA deaminase
Yizhu Lin, Samentha Kwok, Abigail E. Hein, Bao Thai, Yewande Alabi, Megan S Ostrowski, Ke Wu, Stephen N. Floor
The paper introduces REMORA, a method to record RNA-protein interactions on single RNA molecules by using RNA deaminases, and shows competition between PUM1 and PUM2 for RNA binding sites.
Nature Methods · Oct 19, 2023Peer-reviewedMolecular Biology