Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions
Yu Zhao, Camila Coelho, Amanda L. Hughes, Luciana Lazar‐Stefanita, Sandy Yang, Aaron N. Brooks, Roy S. K. Walker, Weimin Zhang, Stephanie L. Lauer, Cindy Hernandez, Jitong Cai, LESLIE ANN MITCHELL, Neta Agmon, Yue Shen, Joseph Sall, , Jef D. Boeke
The paper uses CRISPR D-BUGS and chromosome swapping to identify and resolve genetic interactions in synthetic chromosomes, revealing unexpected links between transcription, inositol metabolism, and tRNA abundance.
Cell · Nov 1, 2023Peer-reviewedGenomics