Author

Stephanie L. Lauer

NYU Langone HealthORCID 0000-0002-6368-9306Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Assay:whole-genome sequencing· 4 Organism:yeast· 4 Research Focus:gene regulatory network inference· 3 Assay:DNA methylation· 2 Research Focus:variant calling· 2 Research Focus:fine-mapping· 1 Assay:long-read sequencing· 1

Frequent Co-Authors

Jef D. BoekeiD· 5 shared Yu ZhaoiD· 5 shared Giovanni StracquadanioiD· 4 shared Joel S. BaderiD· 4 shared LESLIE ANN MITCHELLiD· 4 shared Camila CoelhoiD· 3 shared Jitong Cai· 3 shared Viola FanfaniiD· 3 shared

Activity by Year

2022-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 44 mutual co-author links

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5 shared papersSynthetic Biology

Jef D. BoekeORCID

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

Manipulating the 3D organization of the largest synthetic yeast chromosome

Weimin Zhang, Luciana Lazar‐Stefanita, Hitoyoshi Yamashita, Michael J. Shen, LESLIE ANN MITCHELL, Hikaru Kurasawa, Evgenii Lobzaev, Viola Fanfani, Max A. B. Haase, Xiaoji Sun, Qingwen Jiang, Gregory W. Goldberg, David M. Ichikawa, Stephanie L. Lauer, Laura H. McCulloch, , Jef D. Boeke

This paper synthesizes the largest eukaryotic yeast chromosome, synIV, and investigates how its 3D structure affects gene regulation and transcription.

Molecular Cell · Nov 8, 2023Peer-reviewedSynthetic Biology

Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions

Yu Zhao, Camila Coelho, Amanda L. Hughes, Luciana Lazar‐Stefanita, Sandy Yang, Aaron N. Brooks, Roy S. K. Walker, Weimin Zhang, Stephanie L. Lauer, Cindy Hernandez, Jitong Cai, LESLIE ANN MITCHELL, Neta Agmon, Yue Shen, Joseph Sall, , Jef D. Boeke

The paper uses CRISPR D-BUGS and chromosome swapping to identify and resolve genetic interactions in synthetic chromosomes, revealing unexpected links between transcription, inositol metabolism, and tRNA abundance.

Cell · Nov 1, 2023Peer-reviewedGenomics

Synthetic chromosome fusion: effects on genome structure and function

Jingchuan Luo, Luís A. Vale-Silva, Adhithi R. Raghavan, Guillaume Mercy, Jonna Heldrich, Xiaoji Sun, Mingyu Kenneth Li, Weimin Zhang, Neta Agmon, Kun Yang, Jitong Cai, Giovanni Stracquadanio, Agnès Thierry, Yu Zhao, Camila Coelho, , Jef D. Boeke

The paper synthesizes a shorter yeast chromosome and studies its fusion with other chromosomes, revealing new 3D structural interactions and Red1 binding patterns.

bioRxiv · Aug 10, 2023 · PreprintSynthetic Biology

Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand

Benjamin A. Blount, Xinyu Lu, Maureen R M Driessen, Dejana Jovicevic, Mateo I Sanchez, Klaudia Ciurkot, Yu Zhao, Stephanie L. Lauer, Robert M. McKiernan, Glen-Oliver F. Gowers, Fiachra Sweeney, Viola Fanfani, Evgenii Lobzaev, Kim Palacios-Flores, Roy S. K. Walker, , Tom Ellis

The paper constructs a synthetic yeast chromosome XI and demonstrates how non-coding DNA modifications affect cell fitness, enabling on-demand extrachromosomal circular DNA formation.

bioRxiv · Jul 15, 2022 · PreprintSynthetic Biology

Episomal editing of synthetic constructs in yeast using CRISPR

Yu Zhao, Camila Coelho, Stephanie L. Lauer, Jon M. Laurent, Ran Brosh, Jef D. Boeke

This paper introduces CREEPY, a method for efficiently editing large episomal DNA constructs in yeast, demonstrating its application to a mouse Sox2-harboring episome.

bioRxiv · Jun 21, 2022 · PreprintSynthetic Biology