Neta Agmon

REWRITE enables scar-minimized rewriting of native human loci exceeding 100 kb in hPSCs, demonstrated through synthetic refactoring of the class-I HLA region.

The paper uses CRISPR D-BUGS and chromosome swapping to identify and resolve genetic interactions in synthetic chromosomes, revealing unexpected links between transcription, inositol metabolism, and tRNA abundance.

The paper synthesizes a shorter yeast chromosome and studies its fusion with other chromosomes, revealing new 3D structural interactions and Red1 binding patterns.

The paper transplants the human adenine de novo pathway into yeast to study regulatory mechanisms, finding that PPAT/ADE4 is a central regulatory node influenced by metabolites and mutations.