Camila Coelho

The paper introduces an improved plasmid toolkit to streamline the mSwAP-In method for large-scale genome writing in mammalian cells.

The paper introduces an automated workflow for high-throughput assembly of large DNA libraries using yeast-based methods, achieving high success rates in assembling diverse DNA constructs.

The paper uses CRISPR D-BUGS and chromosome swapping to identify and resolve genetic interactions in synthetic chromosomes, revealing unexpected links between transcription, inositol metabolism, and tRNA abundance.

The paper synthesizes a shorter yeast chromosome and studies its fusion with other chromosomes, revealing new 3D structural interactions and Red1 binding patterns.

The paper uses synthetic regulatory genomics to study Sox2 expression in mESCs, finding that some enhancer regions are context-dependent and can enhance core regulatory elements.

This paper introduces CREEPY, a method for efficiently editing large episomal DNA constructs in yeast, demonstrating its application to a mouse Sox2-harboring episome.