PYR1 biosensor-driven genome-wide CRISPR screens for improved monoterpenoid production in Kluyveromyces marxianus
Nicholas R. Robertson, Chase Lenert-Mondou, Alison C. Leonard, Aida Tafrishi, Stephanie Carrera, Sang Cheon Lee, Yuna Aguilar, Leonardo Sanchez Zamora, Trinity Nguyen, Jesús Beltrán, Mengwan Li, Sean R. Cutler, Timothy A. Whitehead, Ian R. Wheeldon
The paper uses a PYR1 biosensor-driven CRISPR screen in Kluyveromyces marxianus to identify gene knockouts that enhance geraniol production by up to 2-fold.
bioRxiv · Nov 14, 2024 · PreprintSynthetic Biology