Sean R. Cutler

Liverwort MpSDR4 regulates abscisic acid sensitivity and gemma cup growth

This study identifies MpSDR4 in liverworts as a regulator of abscisic acid sensitivity and gemma cup growth, shedding light on the evolutionary divergence of SDR4 function in plant reproduction.

Protein engineering of a genetically encoded biosensor for wastewater detection of profen NSAIDs

The paper engineers a genetically encoded biosensor, PYRNSAID, to detect profen NSAIDs in wastewater with nanomolar sensitivity and demonstrates its functionality in simulated wastewater matrices.

Computational design of dynamic biosensors for emerging synthetic opioids

The paper designs dynamic biosensors for detecting nitazenes and their metabolites using computational methods, achieving low nanomolar detection limits and limited cross-reactivity.

Unusually Broad-spectrum small-molecule sensing using a single protein scaffold

The paper identifies small-molecule sensors using ABA receptors and demonstrates their ability to detect diverse ligands including plant natural products and synthetic chemicals.

Design of post-translational, ligand-controlled inverters and switches

The paper designs and engineers minimal protein-based molecular inverters and switches that respond to ligands, demonstrating tunable and modular control of post-translational signaling.

PYR1 biosensor-driven genome-wide CRISPR screens for improved monoterpenoid production in Kluyveromyces marxianus

The paper uses a PYR1 biosensor-driven CRISPR screen in Kluyveromyces marxianus to identify gene knockouts that enhance geraniol production by up to 2-fold.

Rationalizing diverse binding mechanisms to the same protein fold: in-sights for ligand recognition and biosensor design

This study analyzes two engineered biosensors based on PYR1 to recognize different ligands, revealing binding mechanisms and computational methods for biosensor design.

Robotic Imaging and Machine Learning Analysis of Seed Germination: Dissecting the Influence of ABA and DOG1 on Germination Uniformity

The paper introduces SPENCER, a high-throughput phenotyping platform for seed germination analysis, and investigates the roles of DOG1 and ABA in germination uniformity and dormancy in Arabidopsis.

Systematic characterization of gene function in a photosynthetic organism

The paper systematically characterizes gene function in a photosynthetic organism by analyzing mutant phenotypes across diverse conditions and linking them to functional pathways.