One-step assembly of large CRISPR arrays enables multi-functional targeting and reveals constraints on array design
Chunyu Liao, Fani Ttofali, Rebecca Slotkowski, Steven R. Denny, Taylor D. Cecil, Ryan T. Leenay, Albert J. Keung, Chase L. Beisel
The paper introduces CRATES, a one-step method to assemble large CRISPR arrays, demonstrating its utility for multiplexed gene editing and revealing design constraints.
bioRxiv · May 2, 2018 · PreprintSynthetic Biology