N. Dai

Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein

This paper describes an RNA capping enzyme-T7 RNA polymerase fusion protein that efficiently generates capped synthetic mRNA in a one-step process, achieving up to 95% Cap-1 incorporation.

Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in E. coli

This paper tests 147 restriction enzymes on dZ-modified DNA, finds resistance in 53.1% of cases, and shows dZ incorporation in E. coli plasmids up to 44.3% with co-expression of specific genes.

Biochemical characterization of mRNA capping enzyme from Faustovirus

This study characterizes a single-subunit RNA capping enzyme from Faustovirus, showing it efficiently generates Cap-0 structures and has advantageous properties for synthetic mRNA manufacturing.

GSK-3484862 targets DNMT1 for degradation in cells

This paper shows that GSK-3484862 targets DNMT1 for degradation in cancer cells and mouse embryonic stem cells, leading to DNA hypomethylation without reducing DNMT1 mRNA levels.

RNase H-based analysis of synthetic mRNA 5-prime cap incorporation

The paper describes methods to analyze synthetic mRNA 5' cap incorporation using RNase H-based cleavage and simplified purification techniques.

RNA binding to human METTL3-METTL14 restricts N6-deoxyadenosine methylation of DNA in vitro

This study investigates how the METTL3-METTL14 complex binds and methylates DNA and RNA, revealing distinct preferences for single-stranded DNA and structured RNA.

The structural basis of mRNA recognition and binding by eukaryotic pseudouridine synthase PUS1

This study determines the crystal structure of yeast PUS1 bound to an RNA target, revealing how it recognizes and modifies mRNA and tRNA substrates.

A metal ion orients mRNA to ensure accurate 2'-O ribosyl methylation of the first nucleotide of the SARS-CoV-2 genome

The paper reports structures of the SARS-CoV-2 nsp16/nsp10 enzyme complex before and after RNA product release, revealing conformational changes and the role of a metal ion in 2'-O methylation of the first nucleotide.

Structural Basis of RNA Cap Modification by SARS-CoV-2 Coronavirus

The paper reports the high-resolution structure of SARS-CoV-2 nsp16/nsp10 complex during mRNA cap methylation, revealing conformational changes and a novel ligand-binding site for antiviral targeting.

Using selective lung injury to improve murine models of spatially heterogeneous lung diseases

This paper introduces a method to create spatially heterogeneous lung injury in mice, enabling better study of drug targeting and regeneration in lung diseases.