T. M. Tomasiak

Cryo-EM structure of the tetra-phosphorylated R-domain in Ycf1 reveals key interactions for transport regulation

The paper determines the cryo-EM structure of the tetra-phosphorylated R-domain in Ycf1, revealing key interactions that regulate transport.

Structural Basis for Oxidized Glutathione Recognition by the Yeast Cadmium Factor 1

The paper determines the cryo-EM structure of Ycf1 bound to oxidized glutathione and identifies a 'flex-pocket' mechanism for substrate binding and specificity.

Structural Basis for Autoinhibition by the Dephosphorylated Regulatory Domain of Ycf1

This study determines the structure of dephosphorylated Ycf1 using cryo-EM, revealing an ordered R-domain topology that mediates autoinhibition and provides insights into its phosphorylation-dependent activation mechanism.

The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation

This study presents cryo-EM structures of Ycf1 showing how phosphorylation of the regulatory domain modulates its interaction with NBD1 and affects ATPase activity.

Cryo-EM structure of an elusive pre-transport intermediate of the multidrug transporter BmrCD reveals modes of asymmetric drug binding

Cryo-EM and EPR analysis reveal cooperative substrate binding in BmrCD in an architecture primed for transport.

The peptide sensor motif stabilizes the outward-facing conformation of TmrAB

This study investigates how the peptide sensor motif in TmrAB coordinates conformational changes during ATP-dependent transport, showing it stabilizes the outward-facing state.