T. O. Yeates

Designed fluorescent protein cages as fiducial markers for targeted cell imaging

The paper introduces designed fluorescent protein cages that target specific subcellular locations in mammalian cells for imaging, demonstrating their ability to penetrate cells and be detected with confocal microscopy.

Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion

The paper designs a symmetry-broken tetrahedral protein cage by modifying subunits to display a single copy of an outward-facing domain.

A Suite of Designed Protein Cages Using Machine Learning Algorithms and Protein Fragment-Based Protocols

The paper designs new protein cages using machine learning and fragment-based methods, reporting their structures and insights into assembly success and failure.

Rigidified Scaffolds for 3-Angstrom Resolution Cryo-EM of Small Therapeutic Protein Targets

This paper demonstrates that rigidified scaffolds enable 3-angstrom resolution cryo-EM for small therapeutic proteins like KRAS, overcoming size limitations in structural characterization.

A 3.8 A Resolution Cryo-EM Structure of a Small Protein Bound to a Modular Imaging Scaffold

This paper demonstrates a method to image small proteins using cryo-EM by binding them to a modular scaffold, achieving 3.8 Å resolution for a 26 kDa protein.