R. Castells-Graells

Designed fluorescent protein cages as fiducial markers for targeted cell imaging

The paper introduces designed fluorescent protein cages that target specific subcellular locations in mammalian cells for imaging, demonstrating their ability to penetrate cells and be detected with confocal microscopy.

Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion

The paper designs a symmetry-broken tetrahedral protein cage by modifying subunits to display a single copy of an outward-facing domain.

A Suite of Designed Protein Cages Using Machine Learning Algorithms and Protein Fragment-Based Protocols

The paper designs new protein cages using machine learning and fragment-based methods, reporting their structures and insights into assembly success and failure.

Rigidified Scaffolds for 3-Angstrom Resolution Cryo-EM of Small Therapeutic Protein Targets

This paper demonstrates that rigidified scaffolds enable 3-angstrom resolution cryo-EM for small therapeutic proteins like KRAS, overcoming size limitations in structural characterization.

Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process

This study identifies host processes targeted by Phytophthora infestans RXLR effectors through protein-protein interaction screens, revealing vesicle trafficking as a major effector-targeted process.

A CRISPR/Cas9 mediated 53 kb deletion of the NRC4 gene cluster of tomato does not affect bacterial flagellin-triggered immunity

This study deletes the NRC4 gene cluster in tomato using CRISPR/Cas9 and finds it does not affect flg22-induced immunity.